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PUFiot is a novel high-security crypto co-processor. This highly integrated hardware root-of-trust has integrated NIST-certified cryptographic engines, embedded tRNG, PUF, and secure OTP. It also comprises a standard interface, DMA, memory management unit, and API/FW SDK to help and realize SoC implementation secure operation. PUFiot can not only reduces the costly procedure of key provisioning but also fully supports the silicon supply chain for achieving zero-touch product lifecycle management with inborn native UID, HUK, and attestation key pair and anti-counterfeiting design on hardware, firmware, and software. This webinar starts from the design concept of PUFiot, introduces its applications and comparisons, and will discuss its supply chain management and applications in the next talk.